Quantitative Mass Spectrometry-Based Approaches in Cardiovascular Research

Quantitative mass spectrometry-based approaches in cardiovascular research.

Mass Spectrometry-Based Approaches Toward Absolute Quantitative Proteomics

Mass spectrometry has served as a major tool for the discipline of proteomics to catalogue proteins in an unprecedented scale. With chemical and metabolic techniques for stable isotope labeling developed over the past decade, it is now routinely used as a method for relative quantification to provide valuable information on alteration of protein abundance in a proteome-wide scale. More recently...

Mass spectrometry approaches for vitamin E research.

Vitamin E is an important nutrient with antioxidant and non-antioxidant functions, and certain evidence suggests that it has a cardiovascular protective role. It is therefore important to maintain an optimal vitamin E status. In the present paper, a number of MS applications to monitor vitamin E status and its interactions, including the use of stable-isotope-labelled vitamin E and metabonomics...

FOCUS: MASS SPECTROMETRY-BASED STRATEGIES FOR NEUROPROTEOMICS AND PEPTIDOMICS: RESEARCH ARTICLE Limitations of Mass Spectrometry-Based Peptidomic Approaches

Mass spectrometry-based peptidomic approaches are powerful techniques to detect and identify the peptide content of biological samples. The present study invest igated the l imitat ions of pept idomic approaches using trimethylammonium butyrate isotopic tags to quantify relative peptide levels and Mascot searches to identify peptides. Data were combined from previous studies on human cell lines...

Functional phosphoproteomic mass spectrometry-based approaches

Mass Spectrometry (MS)-based phosphoproteomics tools are crucial for understanding the structure and dynamics of signaling networks. Approaches such as affinity purification followed by MS have also been used to elucidate relevant biological questions in health and disease.The study of proteomes and phosphoproteomes as linked systems, rather than research studies of individual proteins, are nec...